Journal: iScience

Article Title: Temporal transcriptome features identify early skeletal commitment during human epiphysis development at single-cell resolution

doi: 10.1016/j.isci.2023.107200

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Article Snippet: Mouse monoclonal IgG 1 κ Erg-1/2/3 antibody , Santa Cruz , Cat#sc376293; RRID: AB_10989086.

Techniques: Staining, RNA Extraction, Reverse Transcription, Derivative Assay, Software, Imaging

Journal: International Journal of Molecular Sciences

Article Title: Age-Related Memory Impairment Is Associated with Increased zif268 Protein Accumulation and Decreased Rpt6 Phosphorylation

doi: 10.3390/ijms21155352

Figure Lengend Snippet: Behavioral results from the retrieval test in a neutral context (mean and SEM) are shown in Panel ( A ). Panels ( B – H ) show zif268 expression reported as a percentage (mean and SEM) of total DAPI present in the same section. Zif268 was increased in both 15-month-old and 22-month-old animals relative to 3-month-old animals in the medial prefrontal cortex (mPFC) ( B ), the aRSC ( C ), pRSC ( D ), dorsal hippocampus (DH) ( E ), ventral hippocampus (VH) ( F ), LA ( G ), and ventrolateral periaqueductal gray (vlPAG) ( H ). In the pRSC, VH, LA, and vlPAG, 22-month-old animals also showed increased zif268 relative to 15-month-old animals. Asterisks indicate p < 0.05; pound signs indicate p < 0.10. White dots represent individual rats.

Article Snippet: Then, slides were incubated in zif268/Erg-1 antibody (Cell Signaling, 1:500, #4153, Beverly, MA, USA) solution (PBS + 0.3% Triton X + 5% NGS) overnight at 4 °C.

Techniques: Expressing

Journal: International Journal of Molecular Sciences

Article Title: Age-Related Memory Impairment Is Associated with Increased zif268 Protein Accumulation and Decreased Rpt6 Phosphorylation

doi: 10.3390/ijms21155352

Figure Lengend Snippet: Animals were divided into two groups (high freezers and low freezers) based on a median split of performance in the Post-CS (conditional stimulus) period during the retrieval test. Results from the Post-CS period during the retrieval test (mean and SEM) are shown in Panel ( A ), where impaired animals froze less than unimpaired animals. Panels ( B – H ) show zif268 expression reported as a percentage (mean and SEM) of total DAPI present in the same section. Impaired animals had increased zif268 relative to unimpaired in the mPFC ( B ). While there was a trend in the aRSC ( C ), impaired animals showed increased zif268 in the pRSC ( D ). No differences were seen in the DH ( E ), but impaired animals had increased zif268 relative to unimpaired animals in the VH ( F ). This finding held in the LA ( G ) and the vlPAG ( H ). Asterisks indicate p < 0.05; pound signs indicate p < 0.10. White dots represent individual rats.

Article Snippet: Then, slides were incubated in zif268/Erg-1 antibody (Cell Signaling, 1:500, #4153, Beverly, MA, USA) solution (PBS + 0.3% Triton X + 5% NGS) overnight at 4 °C.

Techniques: Expressing

Journal: International Journal of Molecular Sciences

Article Title: Age-Related Memory Impairment Is Associated with Increased zif268 Protein Accumulation and Decreased Rpt6 Phosphorylation

doi: 10.3390/ijms21155352

Figure Lengend Snippet: Scatterplots depicting correlations between phosphorylated Rpt6 and zif268 accumulation in the mPFC ( A ), RSC ( B ), DH ( C ), VH ( D ), LA ( E ), and vlPAG ( F ). Unimpaired animals are represented by gray dots, and impaired animals are represented by white dots.

Article Snippet: Then, slides were incubated in zif268/Erg-1 antibody (Cell Signaling, 1:500, #4153, Beverly, MA, USA) solution (PBS + 0.3% Triton X + 5% NGS) overnight at 4 °C.

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: Age-Related Memory Impairment Is Associated with Increased zif268 Protein Accumulation and Decreased Rpt6 Phosphorylation

doi: 10.3390/ijms21155352

Figure Lengend Snippet: Levels of zif268 expression and pRpt6 in each ROI were averaged throughout the entire brain for each animal. Panels ( A , B ) represent zif268 ( A ) and pRpt6 ( B ) levels with groups constructed based on age group. zif268 levels differ between all age groups ( A ). Both 15-month-old and 22-month-old animals showed less pRpt6 than 3-month-old animals, but did not differ from each other. ( B ) Panels ( C , D ) represent zif268 ( C ) and pRpt6 ( D ) levels with groups constructed based on behavioral performance defined by a median split. Animals with impaired memory performance showed increased zif268 ( C ) and decreased pRpt6 ( D ) relative to unimpaired animals. Asterisks indicate p < 0.05. White dots represent individual rats.

Article Snippet: Then, slides were incubated in zif268/Erg-1 antibody (Cell Signaling, 1:500, #4153, Beverly, MA, USA) solution (PBS + 0.3% Triton X + 5% NGS) overnight at 4 °C.

Techniques: Expressing, Construct

Journal: International Journal of Molecular Sciences

Article Title: Age-Related Memory Impairment Is Associated with Increased zif268 Protein Accumulation and Decreased Rpt6 Phosphorylation

doi: 10.3390/ijms21155352

Figure Lengend Snippet: Schematic of coronal section where dorsal hippocampus (DH) slices were taken at −3.00 mm relative to bregma (Panel A ). Representative images from taken at 20× 3-month-old (Panel B ), 15-month-old (Panel C ), and 22-month-old (Panel D ) animals have DAPI depicted in blue and zif268 depicted in red. Insets of the highlighted region are shown in the bottom right corner for each image.

Article Snippet: Then, slides were incubated in zif268/Erg-1 antibody (Cell Signaling, 1:500, #4153, Beverly, MA, USA) solution (PBS + 0.3% Triton X + 5% NGS) overnight at 4 °C.

Techniques: